Top attune flow cytometer Secrets
Top attune flow cytometer Secrets
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Information-enriched characterization of cell populations. Morphological facts from visuals can incorporate to your richness of flow cytometry data. Such as, the figure displays an normally conventional apoptosis assay employing Annexin V and PI, adding mobile imaging to characterize cells in Just about every population to reveal morphologically unique characteristics. These insights couldn't are already received from multiplex staining on your own.
As samples are obtained over the Attune CytPix Flow Cytometer, the large-speed brightfield digital camera captures and retailers photographs of detected situations at a charge approximately 6,000 images for every second, dependant upon the flow rate and graphic dimensions. For better versatility, Attune Cytometric Software program allows you to change the picture capture frequency as desired and choose a specific gate for image capture, if needed.
Mix acoustic focusing technological innovation with higher-parameter Evaluation for versatile spectral unmixing and conventional workflows.
Learn new insights into your experimental data by visualizing populations using blended fluorescence and morphology knowledge. Our computer software interface wizards allow downsampling, concatenation (merged data files) and dimensionality instruments which are created into the software package for an intuitive, streamlined knowledge Investigation working experience.
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Additionally, it truly is illuminating to see what number of doublets slip as a result of traditional singlet gates, and great to validate the standing of unexpected double-good functions in my Evaluation."
Pair your Attune flow cytometer having an autosampler for enhanced workflow effectiveness, and insert robotic integration for optimum throughput.
Steady graphic high quality even at higher throughput Acoustic concentrating and also a significant-speed digicam combine to picture these T cells regularly at minimal or high flow premiums. Simply regulate target and camera configurations to meet experimental needs.
Early indications of this contamination had originally been dismissed as debris. Backgating to find new populations. Backgating imaged cells on the Attune CytPix Flow Cytometer also lets you use morphological characteristics to find fascinating subpopulations that may not be apparent from flow cytometry facts by itself.
End users can perspective party photographs in serious-time to confirm sample high quality, optimize protocols, and history the morphology of each and every cell inhabitants identified for Examination. Imaging seize also Rewards from acoustic concentrating, which really helps to position the cells so that a sharp, centered impression is obtained.
Audio waves permit specific mobile alignment, causing Excellent information high-quality and ~10X a lot quicker sample flow fees.
We Beforehand demonstrated the power of imaging Motor vehicle-T/Ramos mobile interactions. Enable’s take a look at just the population of greatest curiosity, the double positive situations, To find out more. We could now use prolonged morphological parameters (right here, circularity and skewness of intensity) to additional look at the functions of those populations and refine gating on these situations, raising knowledge robustness.
Leverage the power of Sophisticated know-how and innovation to speed up your discoveries. Have confidence in while in the Attune Flow Cytometer household to deliver the reliability, velocity, and precision that established your perform read more aside.
No clean/no lyse leukocyte Investigation with imaging. Cells had been acquired from 24-hour-old blood by dilution in 1 mM EDTA ( Pro UX/UI permits users to teach AI versions for expanded purposes. (A) The “Teach Product” feature supplies annotation instruments and serious-time design instruction accuracy.
Gating on these prolonged parameters means that you can quickly and properly discover populations of interest and ensure gating approach with little if any manual assessment. The Attune software package presents access to point out-of-the-artwork equipment Mastering analysis instruments inside of an expertly designed person interface, rendering it exceptionally easy to use.
The Attune Xenith cytometer is a large-parameter spectral analyzer, supplying equally spectral unmixing and standard flow abilities for thorough and flexible mobile Investigation.
Agilent Worth Assure guarantees you a minimum of 10 years of instrument use from your date you purchase. Normally, We'll credit rating you Together with the residual value of the program towards an upgraded product.
In this article, we display that by gating methods applying graphic-based mostly quantitative parameters can distinguish interacting cells from coincident occasions to far more accurately examine interacting cells.